Reproducibility readiness
Scored 91 / 100
Read as a narrative review of a topic, without a systematic search, against SANRA.
The manuscript is a comprehensive narrative review that clearly outlines its scope, importance, and conceptual model. It thoroughly synthesizes and evaluates primary literature with specific data points, highlighting existing knowledge gaps and limitations in the field. Statements on ethics, data availability, funding, and competing interests are fully reported, though as a narrative review, it does not detail a formal systematic literature search strategy.
The paper explains why the topic matters
Shown“Multiciliated cells, through the coordinated beating of motile cilia, are vital for moving fluids in the brain and mucus in the airways and in the reproductive organs. Their dysfunction leads to diseases, associated with chronic airway infections, subfertility and ventricular enlargement.”
The paper states the review's aims or questions
Shown“In this review, we propose a model in which mechanical forces act as contextual regulators that license, time and scale the execution of the multiciliated differentiation programme.”
The paper describes how the literature was searched: databases or sources, dates and search terms
MissingThe paper supports its key statements with references
Shown“In vertebrates, centriole amplification pathways involved in generating multiciliated cells are mainly conserved. Progenitor cells, marked by tissue-specific markers (e.g. RC2 for radial glial cells in the brain and p63 for basal cells in the airway), possess two centrioles.”
The paper weighs the strength of the evidence it cites, rather than only listing it
Shown“These seemingly contradictory observations can be reconciled by a stage-specific model in which YAP promotes progenitor identity under high mechanical tension but must be downregulated for terminal multiciliated differentiation.”
The paper presents relevant results of the studies it cites, such as outcomes or numbers
Shown“However, while centriole duplication produces only two new centrioles, amplification results in dozens of centrioles (e.g. around 50 in ependymal cells and up to 300 in the airway cells).”
The paper names the ethics approval for work on people or animals, and the consent of the people studied
Shown“Ethics. This work did not require ethical approval from a human subject or animal welfare committee.”
The paper says where the data behind the results can be found, or why they cannot be shared
Shown“Data accessibility. This article has no additional data.”
The paper says where the analysis code or scripts can be found, or that no custom code was written
Doesn't applyThe paper states who funded the work, or that it had no funding
Shown“Funding. This work was supported by the INSERM, the CNRS, the ENS, the ANR (ANR-20-CE45-0019, ANR-21-CE16-0016 and ANR-22-CE16-0011), the Fondation pour la Recherche Médicale (FRMEQU202103012767), the Fondation Pierre-Gilles de Gennes (FPGG03) and PSL-QLife (ANR-17-CONV-0005).”
The paper states the authors' competing interests, or that there are none
Shown“Conflict of interest declaration. We declare we have no competing interests.”
The paper discusses the limitations of the study
Shown“Still, the linearity of the different molecular players described in this section has not been fully investigated making it complex to associate them with the mechanical forces observed during development. This would require investigating the players at precise developmental timing”